The Improvement of Nutritional Characteristics and Antioxidant Potential of Cowpea Flour Through Germination, Roasting and ItsCombinations

Authors

  • Agus Setiyoko Program Studi Teknologi Hasil Pertanian, Fakultas Agroindustri,Universitas Mercu Buana Yogyakarta, Jl. Wates km 10 Yogyakarta 55753
  • Yuli Perwita Sari Program Studi Teknologi Hasil Pertanian, Fakultas Agroindustri,Universitas Mercu Buana Yogyakarta, Jl. Wates km 10 Yogyakarta 55753

Keywords:

antioxidan, anti-nutritional, owpea flour, pre-treatment

Abstract

Cowpeas are a particular kind of legume and containthe second highest protein after soybeans. Cowpea is inadequate because it has antinutritions such as tannins, phytic acid, and trypsin inhibitors. Pretreatment is necessary to lower anti-nutritional elements and increase the nutrition of cowpeas' ability to be digested. This study was done to determine the effect of various pretreatment techniques on enhancing cowpea flour's nutritional qualities and antioxidant potential. A completely randomized design with one factorial and three analytical replications was done. The findings of this study demonstrate that cowpea flour may be made using a variety of pretreatments, including germination, roasting, or its combination. A combination of germination treatment followed by roasting at 1400C for 40 minutes can significantly reduce the phytic acid, tannin, and color content and increase the yield and density of the cowpea flour produced. In addition, the initial treatment with the combination had the highest protein content of 46.88%, the highest total phenol, and the most increasedRadical Scavenging Activity(RSA) or antioxidant activity of 47.00%. The most effective method to produce cowpea flour withthe best nutritional properties and antioxidant potential is a combination of germination and roasting processes as a pretreatment

References

Ai, N. S., & Ballor, M. (2010). Peranan Air dalam Perkecambahan Biji. Jurnal IlmiahSains, 10(2), 190–195.AOAC. (2005). Official methods of analysis of the Association of Analytical Chemist.Association of Official Analytical Chemist, Inc.Ariviani, S., Sholihin, N. H., & Nastiti, G. P. (2021). Pengembangan Tepung Kecambah Kacang Tunggak (Vigna unguiculata)Sebagai Sereal Fungsional Kaya Serat Pangan dan Berpotensi Antioksidan. Jurnal Teknologi Hasil Pertanian, 14(2), 84. https://doi.org/10.20961/jthp.v14i2.53422Asih, D. J., Kadek Warditiani, N., & Wiarsana, I. G. S. (2022). Aktivitas Antioksidan Ekstrak Amla (Phyllanthus emblica / Emblica officinalis).Humantech, 1, 674–687.Cai, R., Hettiarachchy, N. S., & Jalaluddin, M. (2003). High-Performance Liquid Chromatography Determination of Phenolic Constituents in 17 Varieties of Cowpeas. Journal of Agricultural and Food Chemistry, 51(6),1623–1627. https://doi.org/10.1021/jf020867bDewi, I. G. A. A. S. P., Ekawati, I. G. A., & Pratiwi, I. D. P. K. (2018). Pengaruh Lama Perkecambahan Millet (Panicum milliaceum)Terhadap Karakteristik Flakes. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 7(4), 175. https://doi.org/10.24843/itepa.2018.v07.i04.p04

Elvira, N., Wisaniyasa, N. W., & Hapsari, N. M. I. (2019). StudiSifat Kimia, Fungsional, dan Daya Cerna Protein Tepung Kecambah Kacang Tunggak (Vigna unguiculata (L.) Walp). Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology), 6(1), 43–53.Guvitha, D. R., & Suhartiningsih. (2019). Pengaruh Substitusi Puree Kacang Tunggak(Vigna unguiculataL.Walp) dan Jumlah Air Terhadap Sifat Organoleptik French Baguette (Roti Perancis). Tata Boga, 8(1), 191–201.Islaku, D., Djarkasi, G. S. S., & Oessoe, Y. Y. E. (2017). Pengaruh subtitusi tepung tapioka dantepung sukun (Artocarpus communis) terhadap sifat sensoris dan kimia biskuit. Cocos, 1(7), 1–11.Ismayanti, M., & Harijono. (2015). Formulasi MPASI Berbasis Tepung Kecambah Kacang Tunggak dan Tepung Jagun dengan Metode Linear Programming. Jurnal Pangan Dan Agroindustri, 3(3), 996–1005.Kim, H. G., Kim, G. W., Oh, H., Yoo, S. Y., Kim, Y. O., & Oh, M. S. (2011). Influence of roasting on the antioxidant activity of small black soybean (Glycine max L. Merrill). Lwt, 44(4), 992–998. https://doi.org/10.1016/j.lwt.2010.12.011Kusumaningrum, A., Wayan Gunam, I. B., & Mahaputra Wijaya, I. M. (2019). Optimasi Suhu dan pH Terhadap Aktivitas Enzim Endoglukanase Menggunakan Response Surface Methodoly (RSM) . Jurnal Rekayasa Dan Manajemen Agroindustri, 7(2), 243. https://doi.org/10.24843/jrma.2019.v07.i02.p08Lastari, A. N., Anandito, R. B. K., & Siswanti, S. (2016). Pengaruh konsentrasi Natrium Metabisulfit (Na2S2O5) Dan Lama Perendaman Terhadap Karakteristik Tepung Kecambah Kedelai. Jurnal TeknosainsPangan, 5(2), 1–8.Lestari, P. A., Yusasrini, N. L. A., & Wiadnyani, A. A. I. S. (2019). Pengaruh Perbandingan Terigu dan Tepung Kacang Tunggak Terhadap Karakteristik Crackers. Jurnal Ilmu dan Teknologi Pangan,8(4), 457–464.Mardiana, R., Shidiq,S. S., Widiastuti, E., & Hariyadi, T. (2021). Pengaruh Suhu Roasting Terhadap Perubahan Kadar Lemak, Kadar Asam Total, dan Morfologi Mikrostruktural Kopi Robusta. Prosiding The 12th Industrial Research Workshop and National Seminar,4(5), 151–156.Mardjan, S. S., Heri Purwanto, E., & Yoga Pratama, G. (2022). Pengaruh Suhu

Awal Dan Derajat Penyangraian Terhadap Sifat Fisikokimia Dan Citarasa Kopi Arabika Solok. Jurnal Keteknikan Pertanian, 10(2), 108–122. https://doi.org/10.19028/jtep.010.2.108-122Marsono, Y. , R. S. , dan N. Z. (2005). Antioksidan Dalam Kacang-Kacangan: Aktivitas dan Potensi Serta Kemampuannya Menginduksi Pertahanan Antioksidan Pada Model Hewan Percobaan.Mbah, B. O., Eme, P. E., & Ogbusu, O. F. (2012). Effect of cooking methods (boiling and roasting) on nutrients and anti-nutrients content of Moringa oleifera seeds. Pakistan Journal of Nutrition, 11(3), 211–215. https://doi.org/10.3923/pjn.2012.211.215Mesfin, N., Belay, A., &Amare, E. (2021). Effect of germination, roasting, and variety on physicochemical, techno-functional, and antioxidant properties of chickpea (Cicer arietinumL.) protein isolate powder. Heliyon, 7(9), 1–8. https://doi.org/10.1016/j.heliyon.2021.e08081Moniharapon, E., Nendissa, S. J., & Laiyan, D. (2017). Karakterisasi Sifat Kimia Tepung Kacang Lawa Merah (Phaseolus vulgaris L.) Dengan Beberapa Perlakuan Pendahuluan . AGRITEKNO, Jurnal Teknologi Pertanian, 6(1), 21. https://doi.org/10.30598/jagritekno.2017.6.1.21Naikoo, M. I., Dar, M. I., Raghib, F., Jaleel, H., Ahmad, B., Raina, A., Khan, F. A., & Naushin, F. (2019). Role and regulation of plants phenolics in abiotic stress tolerance: An overview. Plant Signaling Molecules: Role and Regulation under Stressful Environments, 157–168. https://doi.org/10.1016/B978-0-12-816451-8.00009-5Narsih, Agato, & Sesario, R. (2018). Penurunan Senyawa Antinutrisi Pada Biji Jagung Dengan Berbagai Metoda. Jurnal Teknologi Pangan, 9(1), 45–50. https://doi.org/10.35891/tp.v9i1.944Nurmala, I., Rachmawan, O., & Suryaningsih, L. (2014). Pengaruh Metode Pemasakan Terhadap Komposisi Kimia Daging Itik Jantan Hasil Budidaya Secara Intensif. Journal of Animal Science, 3(2), 1–10.Ojha, P., Adhikari, R., Karki, R., Mishra, A., Subedi, U., & Karki, T. B. (2018). Malting and fermentation effects on antinutritional components and functional characteristics of sorghum flour. Food Science and Nutrition, 6(1),

47–53. https://doi.org/10.1002/fsn3.525Olanipekun, O. T., C, O. E., A, O. O., & G, O. O. (2015). Effect of boiling and roasting on the nutrient composition of kidney beans seed flour. Sky Journal of Food Science, 4(2), 24–029.Padmashree, A., Negi, N., Handu, S., Khan, M. A., Semwal, A. D., & Sharma, G. K. (2019). Effect of germination on nutritional, antinutritional and rheological characteristics of chenopodium quinoa. Defence Life Science Journal, 4(1), 55–60. https://doi.org/10.14429/dlsj.4.12202Palungan, M. B., Rapa, C. I., & Salu, S. (2018). Process of Coffee Processing of Arabic Powder and Physical Changes Due To Adjustment Temperature. Prosiding SNTTM XVII, 271–274.Pamungkas, M. T., Masrukan, M., & SAR, K. (2021). Pengaruh Suhu Dan Lama Penyangraian (Roasting) Terhadap Sifat Fisik Dan Kimia Pada Seduhan Kopi Arabika (Coffea ArabicaL.) Dari Kabupaten Gayo, Provinsi Aceh. Agrotech : Jurnal Ilmiah Teknologi Pertanian, 3(2), 1–10. https://doi.org/10.37631/agrotech.v3i2.278Pangastuti, H. A., Affandi, D. R., &Ishartani, D. (2013). Karakterisasi Sifat Fisik dan Kimia Tepung Kacang Merah (Phaseolus vulgaris L.)dengan Beberapa Perlakuan Pendahuluan. Jurnal Teknosains Pangan, 2(1), 20–29. Purnamayanti, Ni. P. A., Ida, B. P., & Gede, A. (2017). Pengaruh Suhu dan Lama Penyangraian Terhadap Karakteristik Fisik dan Mutu Sensori Kopi Arabika (Coffea arabica L). Jurnal BETA (Biosistem Dan Teknik Pertanian, 5(2), 39–48.Puspitasari, D., Rejeki, F. S., Wedowati, E. R., Koesriwulandari, & Kadir, A. (2020). Kualitas Biskuit MP-ASI Dari Tepung Komposit Kimpul-Kacang Tunggak dan Tepung Sagu Selama Penyimpanan. Journal of Research and Technology, 6(1), 70–80.Putri, A., Wisaniyasa, N. W., & Suparthana, I. P. (2021). Pengaruh Lama Perkecambahan Terhadap Total Fenol, Flavonoid, Dan Aktivitas Antioksidan Tepung Kecambah Kacang Tunggak (Vigna unguiculata L. Walp.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 10(1), 36. https://doi.org/10.24843/itepa.2021.v10.i01.p04Putri, D. desmiyeni, Nurmagustina, D. E., & Chandra, Agung, A. (2014). Kandungan total fenol dan aktivitas antibakteri kelopak buah rosela merah dan ungu sebagai kandidat feed

additive alami pada broiler. Jurnal Penelitian Pertanian Terapan, 14(3), 174–180.Safitri, F. M., Ningsih, D. R., Ismail, E., & Waluyo, W. (2016). Pengembangan getuk kacang tolo sebagai makanan selingan alternatif kaya serat. Jurnal Gizi Dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics), 4(2), 71. https://doi.org/10.21927/ijnd.2016.4(2).71-80Salami, K. O., Karim, O. R., Belewu, M. A., & Olapade, G. M. (2020). Effect of pre-treatment methods on the physicochemical and anti-nutritional properties of Bambara groundnut yoghurt. Ceylon Journal of Science, 49(4), 423.https://doi.org/10.4038/cjs.v49i4.7822Saputro, D. H., Andriani, M., & Siswanti. (2015). Karakteristik Sifat Fisik Dan Kimia Formulasi Tepung Kecambah Kacang-Kacangan Sebagai Bahan Minuman. Jurnal Teknosains Pangan, 4(1), 11.Setiarto, R. H. B., &Widhyastuti, N. (2016). Penurunan Kadar Tanin dan Asam Fitat Pada Tepung Sorgum Melalui Fermentasi Rhizopus oligosporus, Lactobacillus plantarum dan Saccharomyces cerevisiae . Berita Biologi, 15(2), 149–157.Sun, J., Wang, P., Zhou, T., Rong, J., Jia, H., & Liu, Z. (2017). Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-07424-0Sutanti, A., Luwihana, S., & Kanetro, B. (2013). Pengaruh Perlakuan Pendahuluan dan Konsentrasi Tepung Kacang Tunggak (Cowpea) Terhadap Sifat Fisik dan Tingkat Kesukaan Oyek. Jurnal AgriSains, 4(7), 11–22.Syukri, D., Sari, F. I. P., & Rini. (2023). Roasting conditions on metabolic profile of black honey arabica coffee (Coffea arabica). IOP Conference Series: Earth and Environmental Science, 1182(1), 012048. https://doi.org/10.1088/1755-1315/1182/1/012048Tamaroh, S., & Sudrajat, A. (2021). Antioxidative Characteristics and Sensory Acceptability of Bread Substituted with Purple Yam (Dioscorea alata L.). International Journal of Food Science, 2021, 1–9. https://doi.org/10.1155/2021/5586316Tursiman, Ardiningsih, P., & Nofiani, R. (2012). TOTAL FENOL FRAKSI ETIL ASETAT DARI BUAH ASAM KANDIS (Garcinia dioica Blume).

Jurnal Kimia Khatulistiwa, 1(1), 45–48.Uchegbu, N. N., & Ishiwu, C. N. (2016). Germinated Pigeon Pea (Cajanus cajan): a novel diet for lowering oxidative stress and hyperglycemia. Food Science and Nutrition, 4(5), 772–777. https://doi.org/10.1002/fsn3.343Utami Dewi, N., Wrasiati, L., & Anom Yuarini, D. (2016). Pengaruh Suhu Dan Lama Penyangraian Dengan Oven Drier Terhadap Karakteristik Teh Beras Merah Jatiluwih. Jurnal Rekayasa Dan Manajemen Agroindustri, 4(2), 1–12.Wahyuni, N. L. E., Rispiandi, R., & Hariyadi, T. (2020). Effect of bean maturity and roasting temperature on chemical content of robusta coffee. IOP Conference Series: Materials Science and Engineering, 830(2). https://doi.org/10.1088/1757-899X/830/2/022019Wijanarti, S., Rahmatika, A. M., & Hardiyanti, R. (2019). Pengaruh Lama Penyangraian Manual Terhadap Karakteristik Kakao Bubuk. Jurnal Nasional Teknologi Terapan (JNTT), 2(2), 212. https://doi.org/10.22146/jntt.42758Wisaniyasa, N. W., & Suter, I. K. (2016). Kajian Sifat Fungsional Dan Kimia Tepung Kecambah Kacang Merah (L . ) Study Of Functional And Chemical Properties Of Red Bean (L.) sprouts flour. Media Ilmiah Teknologi Pangan, 3(1), 26–34.Yasser, M., Rafi, M., Wahyuni, W. T., Widiyanti, S. E., & Asfar, A. M. I. A. (2020). Total phenolic content and antioxidant activities of buni fruit (Antidesma buniusL.) in moncongloe maros district extracted using ultrasound-assisted extraction. Rasayan Journal of Chemistry, 13(1), 684–689. https://doi.org/10.31788/RJC.2020.1315584

Downloads

Submitted

2026-04-26

Published

2026-07-07

How to Cite

Setiyoko, A., & Sari, Y. P. (2026). The Improvement of Nutritional Characteristics and Antioxidant Potential of Cowpea Flour Through Germination, Roasting and ItsCombinations. Agroindustrial Technology Journal, 7(3), 19–37. Retrieved from https://atj.journal.unida.gontor.ac.id/index.php/atj/article/view/67