Traditional Biotechnology Approaches in Halal Food Production: A Comprehensive Review

Authors

  • Ashari Harunaningtyas Food Science, Indonesian School of Pharmacy, Indonesia
  • Nanda Legiasa Rabiul Tsani Rohana Food Science and Technology, Gadjah Mada University, Indonesia

Keywords:

traditional biotechnology, fermentation, halal, microorganism, thayyib

Abstract

The concepts of halaland thayyib arekey considerations in Muslim-majority nations. Halaland thayyib are the highest quality standards, as well as indicators of product competitiveness. This concept can be applied to all productsor services consumed. Traditional biotechnology is one applicationofthehalal and thayyib principle.Traditional biotechnology involves the activity of microorganisms, namely, fermentation. Fermented products include tape, kombucha, yogurt, bread, and tauco. These five products are well known within Muslim communities,hence the need for critical examination of their halal and thayyib aspects. In terms of halal, fermented products must ensure the halalstatus of the raw materials, media used, and food additives. Meanwhile, in terms of thayyib, fermented products must ensure food safety, process hygiene, and the use of high-quality raw materials, thereby allowingMuslim consumerstofeel safe andconfident in consuming these products.

References

Addina, F. N., Santoso, I., & Sucipto. (2020). Concept of halal food development to support halal tourism: A review. IOP Conference Series: Earth and Environmental Science, 475(1). https://doi.org/10.1088/1755-1315/475/1/012053

Affairs, U. N. D. of E. and S. (2024). World Population Prospects 2024. In United Nation(Issue 9).

Aini, N. ’, & Khiftiyah, A. M. (2021). The Potency of Local Fermented Foods Tape Ketan to Improve The Body’s Immune System. Multidiscipline International Conference, 427–431.

Alzeer, J., Rieder, U., & Hadeed, K. A. (2018). Rational and practical aspects of Halal and Tayyib in the context of food safety. Trends in Food Science and Technology, 71(July), 264–267. https://doi.org/10.1016/j.tifs.2017.10.020

Alzeer, J., Rieder, U., & Hadeed, K. A. (2020). Good agricultural practices and its compatibility with Halal standards. Trends in Food Science and Technology, 102, 237–241. https://doi.org/10.1016/j.tifs.2020.02.025

Anuar, F. N., Mamat, M. N., & Tumiran, M. A. (2024). The Importance Of Halalan Tayyiban In Food Consumption Among Adolescents: A Conceptual Analysis. Al-Qanatir: International Journal of Islamic Studies, 33(4), 199–207.

Atma, Y., Taufik, M., & Seftiono, H. (2018). Identifikasi Resiko Titik Kritis Kehalalan Produk Pangan : Studi Produk Bioteknologi. 10(1), 59–66.

Bachtiar, W. F. (2025). Relegitimizing Halal Food and Beverage Violations: The Role of Brand Ethical Behavior and Perceived Brand Integrity.

Badan Pusat Statistik. (2025). Jumlah penduduk pertengahan tahun (ribu jiwa), 2025. https://www.bps.go.id/id/statistics-table/2/MTk3NSMy/jumlah-penduduk-pertengahan-tahun--ribu-jiwa-.html

Castellone, V., Bancalari, E., Rubert, J., Gatti, M., Neviani, E., & Bottari, B. (2021). Eating fermented: Health benefits of lab-fermented foods. Foods, 10(11), 1–22. https://doi.org/10.3390/foods10112639

Cempaka, L. (2021). Peuyeum: fermented cassava from Bandung, West Java, Indonesia. Journal of Ethnic Foods, 8(1), 0–6. https://doi.org/10.1186/s42779-021-00079-3

Chakravorty, S., Bhattacharya, S., Chatzinotas, A., Chakraborty, W., Bhattacharya, D., & Gachhui, R. (2016). Kombucha tea fermentation: Microbial and biochemical dynamics. International Journal of Food Microbiology, 220, 63–72. https://doi.org/10.1016/j.ijfoodmicro.2015.12.015

D’Amico, V., Gänzle, M., Call, L., Zwirzitz, B., Grausgruber, H., D’Amico, S., & Brouns, F. (2023). Does sourdough bread provide clinically relevant health benefits? In Frontiers in Nutrition(Vol. 10). Frontiers Media SA. https://doi.org/10.3389/fnut.2023.1230043

Dysin, A. P., Egorov, A. R., Godzishevskaya, A. A., Kirichuk, A. A., Tskhovrebov, A. G., & Kritchenkov, A. S. (2023). Biologically Active Supplements Affecting Producer Microorganisms in Food Biotechnology: A Review. Molecules, 28(3), 1–28. https://doi.org/10.3390/molecules28031413

Ermis, E. (2017). Halal status of enzymes used in food industry. Trends in Food Science and Technology, 64, 69–73. https://doi.org/10.1016/j.tifs.2017.04.008

Faridah, H. D., & Sari, S. K. (2019). Utilization of Microorganism on the Development of Halal Food Based on Biotechnology. Journal of Halal Product and Research, 2(1), 33. https://doi.org/10.20473/jhpr.vol.2-issue.1.33-43

Faridah, H. D., & Susanti, T. (2018). Polysaccharide As Gelatin Subtitute Material in Halal Drug Delivery System. Journal of Halal Product and Research, 1(2), 15. https://doi.org/10.20473/jhpr.vol.1-issue.2.15-21

Fathnur. (2019). Uji Kadar Alkohol Pada Tapai Ketan Putih (Oryza L.Var Glutinosa Dan Singkong (Manihot Sp.) Melalui Fermentasi Dengan Dosis Ragi Yang Berbeda. Jurnal Agrisistem, 15(2), 71–79.

Fathurrohim, M. F. (2022). Narrative Review: Study of Microbial-Based Tipping Points As Halal Food Products. International Journal Mathla’ul Anwar of Halal Issues, 2(1), 1–5. https://doi.org/10.30653/ijma.202221.39

Freitas, M. (2017). The Benefits of Yogurt, Cultures, and Fermentation. In The Microbiota in Gastrointestinal Pathophysiology(p. 0). https://doi.org/10.1016/B978-0-12-804024-9/00024-0

Heitmann, M., Zannini, E., & Arendt, E. (2018). Impact of Saccharomyces cerevisiae metabolites produced during fermentation on bread quality parameters: A review. Critical Reviews in Food Science and Nutrition, 58(7), 1152–1164. https://doi.org/10.1080/10408398.2016.1244153

Hendarto, D. R., Handayani, A. P., Esterelita, E., & Handoko, Y. A. (2019). Mekanisme Biokimiawi dan Optimalisasi Lactobacillus bulgaricus dan Streptococcus thermophilus dalam Pengolahan Yoghurt yang Berkualitas. J. Sains Dasar, 8(1), 13–19.

Herlina, V. T., Lioe, H. N., Kusumaningrum, H. D., & Adawiyah, D. R. (2022). Nutritional composition of tauco as Indonesian fermented soybean paste. Journal of Ethnic Foods, 9(1). https://doi.org/10.1186/s42779-022-00159-y

Herlina, V. T., Lioe, H. N., Kusumaningrum, H. D., & Adawiyah, D. R. (2024). Low molecular weight peptides in tauco, a fermented soy product, associated to umami taste through peptidomics-sensomics approach. International Journal of Food Science and Technology, 59(5), 3151–3166. https://doi.org/10.1111/ijfs.17060

Hidayat, B., Hasanudin, U., Muslihudin, M., Akmal, S., Nurdjanah, S., & Yuliana, N. (2020). Growth kinetics of Saccharomyces cerevisiae and tape yeast on the cassava pulp fermentation. Journal of Physics: Conference Series, 1500(1). https://doi.org/10.1088/1742-6596/1500/1/012058

Idris, P. S. R. P. H., Musa, S. F. P. D., & Sumardi, W. H. H. (2022). Halal-Tayyiban and Sustainable Development Goals: A SWOT Analysis. International Journal of Asian Business and Information Management, 13(2), 1–16. https://doi.org/10.4018/IJABIM.20220701.oa9

Indasah, I., & Muhith, A. (2020). Local Microorganism from “tape” (Fermented Cassava) in Composition and Its Effect on Physical, Chemical and Biological Quality in Environmental. IOP Conference Series: Earth and Environmental Science, 519(1). https://doi.org/10.1088/1755-1315/519/1/012003

Ismail, W. R. W., Ramli, R. A., Zain, Z. M., Abdullah, H., Zulkifli, A. H., & Azahar, H. (2020). Halal Issues in Pastry and Bakery Products: The Halal Gatekeepers of Bakery Ingredients. https://www.researchgate.net/publication/339089621

Jakubczyk, K., Kupnicka, P., Melkis, K., Mielczarek, O., Walczyńska, J., Chlubek, D., & Janda-Milczarek, K. (2022). Effects of Fermentation Time and Type of Tea on the Content of Micronutrients in Kombucha Fermented Tea. Nutrients, 14(22). https://doi.org/10.3390/nu14224828

Jannah, A. W., Inayatulloh, H. M., Mumtazah, M., & Supriyatna, A. (2023). HALAL CRITICAL POINT ANALYSIS OF SOME TOAST SAMPLES IN CIBIRU DISTRICT , BANDUNG CITY. Journal of Halal Research, Policy and Industry, 2(1).

Jumiono, A., Mardiah, M., Amalia, L., & Puspasari, E. (2024). Identifikasi Titik Kritis Kehalalan Produk Mikrobiologi. Jurnal Ilmiah Pangan Halal, 6(1), 84–95. https://doi.org/10.30997/jiph.v6i1.10633

Karahalil, E. (2020). Principles of halal-compliant fermentations: Microbial alternatives for the halal food industry. Trends in Food Science and Technology, 98(January), 1–9. https://doi.org/10.1016/j.tifs.2020.01.031

Khaerah, A., & Akbar, F. (2019). Aktivitas Antioksidan Teh Kombucha dari Beberapa Varian Teh yang Berbeda. Prosiding Seminar Nasional LP2M UNM, 472–476.

Khotibul Umam, Dyah Purbaningrum, & Iklim Matunasia Aci. (2024). Challenges and Opportunities in Implementing a Halal Lifestyle: A Study of Global Halal Regulations. MONETARIUM: Journal of Economics Business and Management, 1(2), 75–86. https://doi.org/10.71155/v9801q74

Kok, C. R., & Hutkins, R. (2018). Yogurt and other fermented foods as sources of health-promoting bacteria. Nutrition Reviews, 76, 4–15. https://doi.org/10.1093/nutrit/nuy056

Kurniadi, M. (2016). Perspektif Halal Produk Pangan Berbasis Bioproses Mikrobia. Jurnal Universiitas Dipenogoro, 16, 147–160.

Laili, S. N., Sudarti, S., & Prihandono, T. (2022). Analysis of Alcohol Content in Cassava (Tape) Fermentation Process. Journal of Science and Science Education, 3(1), 17–21. https://doi.org/10.29303/jossed.v3i1.1340

Lisko, D. J., Johnston, G. P., & Johnston, C. G. (2017). Effects of dietary yogurt on the healthy human gastrointestinal (Gi) microbiome. Microorganisms, 5(1). https://doi.org/10.3390/microorganisms5010006

Ma’rifat, T. N., Purwanto, S., & Windarwati, S. (2017). Perception on Halal Traceability on Chicken Meat Supply Chain. Agroindustrial Technology Journal, 1(1), 33–42.

Mazalan, H. B., Rambli, M. M., Lim, S. A., & Nore, B. F. (2023). Development of Halalan Tayyiban Plant-based Cheese Formulations. ASEAN Journal on Science and Technology for Development , 40(3), 181–187. https://doi.org/10.61931/2224-9028.1543

Mollakhalili-Meybodi, N., Khorshidian, N., Nematollahi, A., & Arab, M. (2021). Acrylamide in bread: a review on formation, health risk assessment, and determination by analytical techniques. Environmental Science and Pollution Research, 28, 15627–15645. https://doi.org/10.1007/s11356-021-12775-3/Published

Nawawi, N. S. M., Abdullah, N., Noor, Z. M., & Bujang, A. (2018). A Review on Food Safety Risk in Bakery Outlet: Halalan-Toyyiban Perspective BT-Proceedings of the 3rd International Halal Conference (INHAC 2016)(N. Muhammad Hashim, N. N. Md Shariff, S. F. Mahamood, H. M. Fathullah Harun, M. S. Shahruddin, & A. Bhari, Eds.; pp. 533–543). Springer Singapore.

Nst, R. H., Idris, M., Azizah, N., Hardiyansah, Y., Damanik, A. M., Harnum, S. S., Syah, F., & Wulandari, D. (2025). Pembuatan dan Potensi Tape Sebagai Produk Fermentasi Tradisional Untuk Kesehatan. Jurnal Pengabdian Pada Masyarakat, 2.

Nurmilah, S., Frediansyah, A., Cahyana, Y., & Utama, G. L. (2024). Biotransformation and health potential of isoflavones by microorganisms in Indonesian traditional fermented soy products: A review. Journal of Agriculture and Food Research, 18(September), 101365. https://doi.org/10.1016/j.jafr.2024.101365

Nurul Farhanah Hamdan, & Norkhairiah Hashim. (2022). Practices of Halalan Thayyiban Aspect in Japanese Restaurants in Brunei Darussalam. Journal of Halal Science and Technology, 1(1), 67–73. https://doi.org/10.59202/jhst.v1i1.456

Nyhan, L. M., Lynch, K. M., Sahin, A. W., & Arendt, E. K. (2022). Advances in Kombucha Tea Fermentation: A Review. In Applied Microbiology(Vol. 2, Issue 1, pp. 73–103). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/applmicrobiol2010005

Pauzi, R. Y., & Astuti, D. I. (2025). Exploring Microbial Succession in Indonesian Fermented Soybean Paste (Tauco) through Culture-Dependent Approach. Microbiology and Biotechnology Letters, 53(1), 57–67. https://doi.org/10.48022/mbl.2411.11002

Purnasari, N., & Rusdan, I. H. (2023). Halal Fermented Functional Food in Indonesia : A Review. HalalSphere, 3(2), 59–66. https://doi.org/https://doi.org/10.31436/hs.v3i2.73

Rashwan, A. K., Osman, A. I., & Chen, W. (2023). Natural nutraceuticals for enhancing yogurt properties: a review. Environmental Chemistry Letters, 21(3), 1907–1931. https://doi.org/10.1007/s10311-023-01588-0

Riswanto, D., & Rezaldi, F. (2021). Kombucha Tea: a Study on the Halal of Fermented Drinks. International Journal Mathla’ul Anwar of Halal Issues, 1(2), 71–77. https://doi.org/10.30653/ijma.202112.28

Rosida, F. D., Sudaryati, H. P., & Nur Apriliyanti, F. (2014). Karakteristik Fisiko Kimia dan Aktivitas Antioksidan Tauco Lamtoro Gung (Leucaena leucocephala) Angkak (Charcteristic physico-chemical and antioxidant activity of Leucaena leucocephala-red yeast rice tauco). J.Rekapangan, 8(2), 156–164.

Rossi, E., Efendi, R., Rahmayuni, & Reval, O. (2022). Effect of storage time of halal peptone at refrigerator temperature on bacterial growth. IOP Conference Series: Earth and Environmental Science, 1059(1). https://doi.org/10.1088/1755-1315/1059/1/012055

Savaiano, D. A., & Hutkins, R. W. (2021). Yogurt, cultured fermented milk, and health: A systematic review. Nutrition Reviews, 79(5), 599–614. https://doi.org/10.1093/nutrit/nuaa013

Seveline, S., Dewanti-Hariyadi, R., Nuraida, L., & Ananta Kusuma, W. (2025). Tauco, fermented Indonesian soybean, processing and the nutritional value. BIO Web of Conferences, 169. https://doi.org/10.1051/bioconf/202516902002

Siregar, M. L., & Zahradika, A. (2023). Halal Traceability System (Hts) Untuk Integrasi Halal Meat Supply Chain (Hmsc) Terhadap Proses Industri Daging Ayam Pada Rumah Potong Hewan.

Sucipto, S., Damayanti, R. W., Perdani, C. G., Kamal, M. A., Astuti, R., & Hasanah, N. (2022). Decision Tree of Materials: A Model of Halal Control Point (HCP) Identification in Small-Scale Bakery to Support Halal Certification. International Journal of Food Science, 2022. https://doi.org/10.1155/2022/5244586

Talib, Z., Rahman, N. A., Iskandar, M. L., & Kassim, N. (2020). Factors Influence Sustainability in Quality and Halal Integrity among Halal Food Manufacturers in Malaysia during Covid-19 Pandemic.Solid State Technology, 63(4), 2933-2949.

Villarreal-Soto, S. A., Beaufort, S., Bouajila, J., Souchard, J. P., & Taillandier, P. (2018a). Understanding Kombucha Tea Fermentation: A Review. In Journal of Food Science(Vol. 83, Issue 3, pp. 580–588). Blackwell Publishing Inc. https://doi.org/10.1111/1750-3841.14068

Villarreal-Soto, S. A., Beaufort, S., Bouajila, J., Souchard, J. P., & Taillandier, P. (2018b). Understanding Kombucha Tea Fermentation: A Review. Journal of Food Science, 83(3), 580–588. https://doi.org/10.1111/1750-3841.14068

Wahyuningsih, S., Trisnawati, A., Hidayati, N. R., & Lestari, A. D. (2023). Kajian Fisikokimia Yoghurt Beet (Beta Vulgaris) sebagai Inovasi Pangan Diet Sehat. Agroindustrial Technology Journal, 7(2), 65–73. https://doi.org/http://dx.doi.org/10.21111/atj.v7i1.9451

Wistiana, D., & Zubaidah, E. (2015). Karakteristik Kimiawi dan Mikrobiologis Kombucha dari Berbagai Daun Tinggi Fenol Selama Fermentasi. Jurnal Pangan Dan Agro Industri, 3(4), 1446–1457.

Zahrandika, A., & Permadi, D. (2022). Pemetaan Kehalalan Produk Pelaku UMKM Kecamatan Sukasari Kota

Bandung.

Zain, M. Z. M., Shori, A. B., & Baba, A. S. (2022). Potential functional food ingredients in bread and their health benefits. In Biointerface Research in Applied Chemistry(Vol. 12, Issue 5, pp. 6533–6542). AMG Transcend Association. https://doi.org/10.33263/BRIAC125.65336542

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Submitted

2026-01-20

Published

2025-11-30

How to Cite

Harunaningtyas, A., & Rohana , N. L. R. T. (2025). Traditional Biotechnology Approaches in Halal Food Production: A Comprehensive Review. Agroindustrial Technology Journal, 9(2), 42–57. Retrieved from https://atj.journal.unida.gontor.ac.id/index.php/atj/article/view/5